Dog Interleukin 2,IL-2 ELISA Kit

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產品詳情

產品描述：

犬白介素2(IL-2)酶聯免疫試劑盒（CSB-E11258c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL2含量。IL2是重要的免疫細胞因子，其背景在于它能促進T細胞等免疫細胞生長和分化。研究機制上，IL2與受體結合，激活下游信號通路，調控免疫細胞功能，增強機體免疫應答，在腫瘤免疫治療、自身免疫病研究等領域都是關鍵靶點。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于獸醫科研領域對犬類免疫相關疾病的機制研究，如感染性疾病模型建立、疫苗免疫效果評估、炎癥反應動態監測等實驗場景。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

IL2Interleukin-2 ELISA Kit; IL-2 ELISA Kit; T-cell growth factor ELISA Kit; TCGF ELISA Kit
縮寫：

IL2
Uniprot No.：

Q29416
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	81-89
1:2	Average %	95
	Range %	92-98
1:4	Average %	86
	Range %	83-90
1:8	Average %	97
	Range %	94-100

回收率：

The recovery of dog IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	82-90
EDTA plasma (n=4)	105	101-109

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
基因功能參考文獻：
1. immunoreactivities of inflammatory cytokines, such as interleukin (IL)-2, its receptor (IL-2R), IL-4 and its receptor (IL-4R) in the cervical and lumbar spinal cord of young adult and aged beagle dogs, were compared. PMID: 23605681
2. In dogs with leishmaniasis, there was high expression of genes encoding IL-2 in lymph nodes. PMID: 21511273
亞細胞定位：

Secreted.
蛋白家族：

IL-2 family
數據庫鏈接：

KEGG: cfa:403989

STRING: 9615.ENSCAFP00000005941

UniGene: Cfa.7