Chicken Ghrelin ELISA Kit

產品詳情

文獻引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

雞饑餓激素酶聯(lián)免疫試劑盒（CSB-E14230C）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Ghrelin含量。Ghrelin是一種重要靶點。它是胃內產生的肽，能調節(jié)食欲和能量穩(wěn)態(tài)。研究機制方面，Ghrelin與受體結合后，作用于神經內分泌系統(tǒng)，影響下丘腦信號通路，調節(jié)攝食、生長激素釋放等，在肥胖、代謝疾病等研究中受關注。試劑盒檢測范圍為25 pg/mL-400 pg/mL，適用于家禽生理學研究、營養(yǎng)代謝實驗、生長調控機制探索等科研領域，如評估飼料成分對Ghrelin分泌的影響或分析激素水平與生長性能的關聯(lián)性，為禽類生物學研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

Ghrelin
種屬：

Gallus gallus (Chicken)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

25 pg/mL-400 pg/mL
靈敏度：

25 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken ghrelin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-96
1:2	Average %	90
	Range %	86-95
1:4	Average %	95
	Range %	90-100
1:8	Average %	97
	Range %	92-103

回收率：

The recovery of chicken ghrelin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-98
EDTA plasma (n=4)	93	88-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.