Canine Interferon alpha-1/2 ELISA kit

Canine Interferon alpha-1/2 ELISA kit

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中文名稱：

犬α-1/2干擾素(IFN-α1/2)酶聯免疫試劑盒
貨號：

CSB-E09849c
規格：

96T/48T
價格：

￥3800/￥2500
促銷：
其他：

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產品描述：

犬α-1/2干擾素(IFN-α1/2)酶聯免疫試劑盒（CSB-E09849c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的IFN-A1/2含量。IFN-A1/2屬干擾素家族，在機體免疫防御中起關鍵作用。研究發現其能激活細胞內信號通路，誘導抗病毒蛋白表達以抵御病毒感染。還參與調節免疫細胞活性和功能，在對抗腫瘤及自身免疫病中展現潛在價值，是研究熱點。試劑盒檢測范圍為12.5 pg/mL-800 pg/mL，適用于科研場景中犬類I型干擾素的功能研究；其動態水平變化常被用于評估病毒感染模型、疫苗免疫原性實驗或免疫調節相關機制研究；細胞培養上清液可分析體外刺激后免疫細胞分泌情況，組織勻漿則適用于疾病模型中局部干擾素水平的定量分析；為動物免疫學、病毒學及炎癥機制研究提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Interferon alpha-1/2 ELISA Kit; IFN-alpha-1/2 ELISA Kit
縮寫：

IFN-α1/2
Uniprot No.：

P81255
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

12.5 pg/mL-800 pg/mL
靈敏度：

3.12 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine IFN-α1/2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	86-99
1:2	Average %	100
	Range %	94-109
1:4	Average %	92
	Range %	89-96
1:8	Average %	87
	Range %	83-96

回收率：

The recovery of canine IFN-α1/2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-96
EDTA plasma (n=4)	94	88-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	2.815	2.824	2.820	2.726
400	2.417	2.425	2.421	2.327
200	1.834	1.863	1.849	1.755
100	1.042	1.112	1.077	0.983
50	0.518	0.563	0.541	0.447
25	0.248	0.269	0.259	0.165
12.5	0.126	0.131	0.129	0.035
0	0.091	0.096	0.094

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
亞細胞定位：

Secreted.
蛋白家族：

Alpha/beta interferon family
數據庫鏈接：

KEGG: cfa:100685213

UniGene: Cfa.4550

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.