Bovine Fibronectin,FN ELISA Kit

說明書下載

產品詳情

產品描述：

牛纖連蛋白(FN)酶聯免疫試劑盒（CSB-E13781B）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿、細胞裂解物樣本中的FN1含量。FN1即纖連蛋白 1，是細胞外基質的重要成分，在細胞黏附、遷移、增殖等過程中發揮關鍵作用。研究顯示，其異常表達與腫瘤的發生、發展及轉移密切相關。深入探究 FN1 的作用機制，有望為腫瘤等疾病的治療提供新靶點和思路。試劑盒檢測范圍為0.137 μg/mL-100 μg/mL，科研人員可將其應用于細胞外基質功能研究、細胞粘附機制探索、組織再生模型構建或炎癥相關疾病模型的分子機制分析，例如評估體外培養細胞分泌FN的水平，或分析特定病理狀態下組織微環境中FN的動態變化。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

FN1Fibronectin ELISA Kit; FN) [Cleaved into: Anastellin] ELISA Kit
縮寫：

FN1
Uniprot No.：

P07589
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates, cell lysates
檢測范圍：

0.137 μg/mL-100 μg/mL
靈敏度：

0.137 μg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine FN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	92
	Range %	85-102
1:10	Average %	97
	Range %	91-110
1:20	Average %	89
	Range %	83-96
1:40	Average %	95
	Range %	84-100

回收率：

The recovery of bovine FN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	88-105
EDTA plasma (n=4)	97	92-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.; Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
亞細胞定位：

Secreted, extracellular space, extracellular matrix.
組織特異性：

Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix.
數據庫鏈接：

STRING: 9913.ENSBTAP00000010925

UniGene: Bt.23418